{"id":1600,"date":"2023-12-07T18:09:43","date_gmt":"2023-12-07T18:09:43","guid":{"rendered":"https:\/\/chemeng.iisc.ac.in\/symposium\/?page_id=1600"},"modified":"2025-01-21T13:54:13","modified_gmt":"2025-01-21T13:54:13","slug":"karan-saxena","status":"publish","type":"page","link":"https:\/\/chemeng.iisc.ac.in\/symposium\/karan-saxena\/","title":{"rendered":"Karan Saxena"},"content":{"rendered":"<p><strong><span style=\"font-size: 18pt;\">Novel Chromogenic Peroxidase Substrates Substantially Enhance the Sensitivity of Enzymatic Lateral Flow Immunoassays<\/span><\/strong><\/p>\n<p>Lateral flow immunoassays (LFIAs or LFAs) have become indispensable in point-of-care medical diagnostics, but improving their sensitivity remains a critical challenge. In this work, we present a strategy to enhance LFA sensitivity by introducing novel oxidative coupling-based chromogenic substrates for horseradish peroxidase (HRP)-based enzymatic sensitivity enhancement. Motivated by the requirement for more sensitive and cost-effective LFAs, we developed substrates inspired by the chemistry of permanent oxidative hair dyes. These substrates consist of two components: a primary intermediate and a coupler that undergo an oxidative coupling reaction in the presence of H2O2, catalyzed by HRP, to generate an intensely colored dye. LFAs employing these substrates outperformed conventional substrates (3,3\u2032-diaminobenzidine (DAB) and 3,3\u2032,5,5\u2032-tetramethylbenzidine (TMB)) and achieved an unprecedented sub-ng\/mL limit of detection (LoD) for human IgG as a model analyte. LFAs employing these novel substrates could detect human IgG at 0.16\u202fng\/mL (1.07 pM), significantly surpassing reported sensitivities in the literature. These LFAs also exhibit over 40 times improved sensitivity compared to standard gold nanoparticle-based LFAs. Moreover, these substrates are about 10,000X more cost-effective than their conventional counterparts, thus making them a compelling choice for point-of-care diagnostics in low-resource settings. [1]<\/p>\n<p>Keywords: Biosensors; Global Health; Paper-based microfluidics; Point-of-care diagnostics; Rapid Diagnostic Tests (RDTs)<\/p>\n<p>Reference:<br \/>\n[1] Karan Saxena, Bhushan J. Toley,<br \/>\nAlternative chromogenic substrates for horseradish peroxidase-enhanced lateral flow immunoassays provide higher sensitivity at lower cost, Sensors and Actuators B: Chemical, Volume 426, 2025, 137017, ISSN 0925-4005, https:\/\/doi.org\/10.1016\/j.snb.2024.137017.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Novel Chromogenic Peroxidase Substrates Substantially Enhance the Sensitivity of Enzymatic Lateral Flow Immunoassays Lateral flow immunoassays (LFIAs or LFAs) have become indispensable in point-of-care medical diagnostics, but improving their sensitivity remains a critical challenge. In this work, we present a strategy to enhance LFA sensitivity by introducing novel oxidative coupling-based chromogenic substrates for horseradish peroxidase (HRP)-based enzymatic sensitivity enhancement. Motivated [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/chemeng.iisc.ac.in\/symposium\/wp-json\/wp\/v2\/pages\/1600"}],"collection":[{"href":"https:\/\/chemeng.iisc.ac.in\/symposium\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/chemeng.iisc.ac.in\/symposium\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/chemeng.iisc.ac.in\/symposium\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/chemeng.iisc.ac.in\/symposium\/wp-json\/wp\/v2\/comments?post=1600"}],"version-history":[{"count":3,"href":"https:\/\/chemeng.iisc.ac.in\/symposium\/wp-json\/wp\/v2\/pages\/1600\/revisions"}],"predecessor-version":[{"id":2018,"href":"https:\/\/chemeng.iisc.ac.in\/symposium\/wp-json\/wp\/v2\/pages\/1600\/revisions\/2018"}],"wp:attachment":[{"href":"https:\/\/chemeng.iisc.ac.in\/symposium\/wp-json\/wp\/v2\/media?parent=1600"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}