{"id":1953,"date":"2025-01-17T18:16:30","date_gmt":"2025-01-17T18:16:30","guid":{"rendered":"https:\/\/chemeng.iisc.ac.in\/symposium\/?page_id=1953"},"modified":"2025-01-17T18:16:30","modified_gmt":"2025-01-17T18:16:30","slug":"ankit-2","status":"publish","type":"page","link":"https:\/\/chemeng.iisc.ac.in\/symposium\/ankit-2\/","title":{"rendered":"Ankit"},"content":{"rendered":"<p><strong><span style=\"font-size: 18pt;\">Development of a 3D Paper Scaffold for Chemical Gradient <\/span><\/strong><strong><span style=\"font-size: 18pt;\">Generation in Solid Tumor Models <\/span><\/strong><\/p>\n<p>This research presents the development of an innovative paper-supported tumor tissue model incorporating the human breast cancer cell line, MDA-MB-231. Collagen and Matrigel served as the extracellular matrix, suspending the cancer cells to create a biologically relevant microenvironment. The cell-laden matrix was distributed onto ten individual paper discs, which were meticulously stacked to construct a three-dimensional architecture within a custom 3D-printed device. Following a 24\u201348-hour culture period, the layers were de-stacked to evaluate the establishment of biochemical gradients by assessing live\/dead cell percentages, HIF-1\u03b1 expression levels, and the extent of apoptosis across the layers. Notably, a progressive increase in cell death, apoptosis, and HIF-1\u03b1 expression was observed from the top to the bottom layers, reflecting the diffusion-limited conditions of the tumor milieu. These findings underscore the potential of this paper-based tumor model as a sophisticated platform for replicating tumor microenvironments and advancing cancer research.<\/p>\n<p><strong>Keywords<\/strong><br \/>\nPaper-supported tumor model, breast cancer, MDA-MB-231, collagen, Matrigel, 3D culture, tumor microenvironment, diffusion limitations, HIF-1\u03b1, apoptosis, live\/dead cell assay, cancer progression.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Development of a 3D Paper Scaffold for Chemical Gradient Generation in Solid Tumor Models This research presents the development of an innovative paper-supported tumor tissue model incorporating the human breast cancer cell line, MDA-MB-231. Collagen and Matrigel served as the extracellular matrix, suspending the cancer cells to create a biologically relevant microenvironment. The cell-laden matrix was distributed onto ten individual [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/chemeng.iisc.ac.in\/symposium\/wp-json\/wp\/v2\/pages\/1953"}],"collection":[{"href":"https:\/\/chemeng.iisc.ac.in\/symposium\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/chemeng.iisc.ac.in\/symposium\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/chemeng.iisc.ac.in\/symposium\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/chemeng.iisc.ac.in\/symposium\/wp-json\/wp\/v2\/comments?post=1953"}],"version-history":[{"count":1,"href":"https:\/\/chemeng.iisc.ac.in\/symposium\/wp-json\/wp\/v2\/pages\/1953\/revisions"}],"predecessor-version":[{"id":1954,"href":"https:\/\/chemeng.iisc.ac.in\/symposium\/wp-json\/wp\/v2\/pages\/1953\/revisions\/1954"}],"wp:attachment":[{"href":"https:\/\/chemeng.iisc.ac.in\/symposium\/wp-json\/wp\/v2\/media?parent=1953"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}